Experiment 16 : Cellular Respiration

Objective

- To demonstrate redox reaction in cellular respiration


Observation

Question

1. Explain what you understand bt redox reactions.



2, What is the substance in a living cell that has the same function as methylene blue?

Coenzyme, example as vitamin and NAD+

3. Explain the biochemical process based on the observation in each boiling tube.

Boiling tube A : respiration by yeast, methelyne blue is reaction to become decolurised light blue. When tube A was plugged with a cork and was shake, the abundance of oxygen oxidized the methylene blue form light blue to blue. When the stopper is removed yeast undergoes aerobic respiration, reduction occurs to a light white.

Boiling tube B :  respiration by yeast, methelyne blue is reaction to become decolurised light blue. When tube B was plugged with a cork and was shake, the abundance of oxygen oxidised the methylene blue form light blue to blue. After second incubation the suspension remained the same because boiling the enzyme.

Boling tube C:  boling denatured the enzyme.