Experiment 4 : Transport Across Membrane

Objektif

1. to determine the sucrose concentration which is isotonic to potato cells
2. to determine the osmotic pressure of potato cells in atmospheric unit
3. to determine the sodium chloride concentration that cause haemolysis and creanation

Exercise 4.1 : Osmotic pressure of potato cells

Exercise 4.2 : Heamolysis and creanation of blood cells

Shrink

Swollen

Must refer to your result because different student showed different result, the intacellular constituent of the erythrocytes is different individually



Questions

1. a) What is the concentration of NaCl for haemolysis to occur?
    b) Explain your answer

must refer to your result (less or equal to 0.4%, different student showed different result)
because the solution is clear at that concentration, erythrocytes have burst, therefore no erythrocyte can be seen in that solution

2. a) What is the concentration of NaCl for crenation to occur?
    b) Explain your answer

must refer to your result (more or equal to 0.6%, different student showed different result)
because the solution is cloudy, erythrocytes have crenated or shrink.